Characterization of oocyte retrieval cycles with empty zona pellucida

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Characterization of oocyte retrieval cycles with empty zona pellucida

Purpose To identify the factors that characterize cycles with empty zona pellucida (EZP). Methods Thirty-six oocyte retrieval cycles from which EZP were collected and another 36 cycles from which no EZP was collected were compared. The patients were divided into three groups: those with no EZP collected during any cycle, those with EZP collected during all cycles, and those experiencing cycle...

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Availability of Empty Zona Pellucida for Generating Embryonic Chimeras

In the present study we used an empty zona pellucida derived from hatched blastocysts as an alternative source for embryo aggregation and compared results with the conventional microwell method. Denuded 4-cell stage porcine embryos were aggregated by introduction into an empty zona or placement within a concave microwell. The present study showed that although the rate of aggregate formation wa...

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Characterization of human zona pellucida glycoproteins.

The human egg may only be fertilized by one spermatozoon to prevent polyploidy. In most mammals, the primary block to polyspermy occurs at the zona pellucida (ZP). Little is known of the human ZP and the changes occurring following fertilization to prevent polyploidy. Using antibodies directed against synthetic peptides predicted from the human ZP2 and ZP3 cDNA, we identified ZP3 as a 53-60 kDa...

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1 Zona Pellucida Glycoproteins

Abbreviations: ZP, zona pellucida; ZPG, ZP glycoprotein; VE, vitelline envelope; VEG, VE glycoprotein; mZP, mouse ZP; hZP, human ZP; SS, signal sequence; CTP, C-terminal propeptide; CFCS, consensus furin cleavage-site; TMD, transmembrane domain; ZPD, ZP domain; NTS, N-terminal subdomain; CTS, C-terminal subdomain; EHP, external hydrophobic patch; IHP, internal hydrophobic patch; CP, charged pat...

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Interaction of human spermatozoa with the zona pellucida of oocyte: development of the acrosome reaction.

Mammalian spermatozoa are not able to fertilize an oocyte upon ejaculation. To gain fertilizing ability, spermatozoa must, either in vivo or in vitro, undergo a process termed capacitation. Since a reliable marker for capacitation does not exist, it is considered that this process is completed when the spermatozoa are able to undergo acrosomal x intestinal mucosa which might require tandem sign...

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ژورنال

عنوان ژورنال: Reproductive Medicine and Biology

سال: 2017

ISSN: 1445-5781

DOI: 10.1002/rmb2.12071